Analysis of Leu aminoacyl synthetase.

Analysis performed on 2024-07-10 11:39:35

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 48609 824 47785 26569
Archaea 3800 118 3682 1422
Vertebrata 1733 463 1270 501
Mammals 885 228 657 134

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
1176 28 902

Domains

Domains
Current Study 1063, 1177
Guo et al (2010) 1064, 1176

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 1061 115 1176 9.226087
Number of Variants 829 101 930 8.207921
Number of Pathogenic Variants 26 2 28 13.000000
Number of Benign/Unknown Variants 803 99 902 8.111111
Fisher exact test 0.759457

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 1063 113 1176 9.407080
Number of Variants 830 100 930 8.300000
Number of Pathogenic Variants 26 2 28 13.000000
Number of Benign/Unknown Variants 804 98 902 8.204082
Fisher exact test 0.759354

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 1061 115 0.81 (0.00) 0.81 (0.02) 7.763e-01 8.206e-01 60189.50 5.94e-01
Guo et al (2010) 1063 113 0.81 (0.00) 0.81 (0.02) 7.763e-01 8.206e-01 59008.50 6.20e-01

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains